We welcome you to the "2nd Edition of Global Conference on Biofuels and Bioenergy", scheduled from September 11-13, 2025 in Valencia, Spain, offering a unique hybrid experience both in-person and online, ensuring global participation and collaboration with the theme "Advancing Biofuel Research: Bridging the Gap between Lab and Industry ". Biofuels 2025 serves as a vital platform for researchers, academics, industry professionals, and policymakers to converge and exchange insights, innovations, and advancements in the realm of biofuels and bioenergy. With an extensive array of topics spanning from biorefineries to the sustainability of biofuels, our agenda encompasses the entire spectrum of this rapidly evolving field. Attendees will delve into discussions on various generations of biofuels, exploring their production processes, applications, and environmental impacts. Furthermore, the conference will spotlight emerging trends such as biochar, bio-based chemicals, and the utilization of microbial genetics for enhanced biofuel production.